Thermo Fisher Scientific JAM-A (CD321) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 28-238 of human JAM-A/CD321/F11R (NP_0586421) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:JAM-A (CD321),uniProtId:Q9Y624-1,ncbiNodeId:9606,antigenRange:28-238,antigenLength:299,antigenImageFileName:PA5-120157_JAM-A_CD321_Q9Y624-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120157_JAM-A_CD321_Q9Y624-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.64 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913729

Product Specific Information

Positive test controls include: HepG2, SKOV3, Mouse lung, Rat lung. The target is usually found in the following locations: Cell junction, Cell membrane, Single-pass type I membrane protein, tight junction.

Immunogen sequence: SVTVHSSEPE VRIPENNPVK LSCAYSGFSS PRVEWKFDQG DTTRLVCYNN KITASYEDRV TFLPTGITFK SVTREDTGTY TCMVSEEGGN SYGEVKVKLI VLVPPSKPTV NIPSSATIGN RAVLTCSEQD GSPPSEYTWF KDGIVMPTNP KSTRAFSNSS YVLNPTTGEL VFDPLSASDT GEYSCEARNG YGTPMTSNAV RMEAVERNVG V

Target Information

CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins. CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, stem cells, epithelial and endothelial cells, and plays a role in mediating cell-cell contact. CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin. CD321 is involved in the maintenance of epithelial and endothelial barriers, hematopoiesis, angiogenesis, and tumor proliferation, invasion, and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.