Thermo Fisher Scientific INSR alpha Monoclonal Antibody (83-7)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody is specific for insulin receptor (IR) and shows no cross-reactivity with insulin-like growth factor (IGF) receptors.
The epitope for this monoclonal antibody is conformational and located in exon 3.

Staining Recommendation:
Formalin-fixed, paraffin-embedded tissues require digestion with pepsin (1 mg/mL in Tris-HCl, pH 2.0) for 15 min at room temperature or 10 min at 37°C.
Recommended positive controls include IM-9 lymphocytes, placenta, or breast carcinoma.

Target Information

Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways via phosphorylation cascades.
Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These receptors differ in sequence regions conferring ligand specificity and intracellular signaling domains, leading to distinct functional outcomes.
Defects in IR are associated with insulin resistance syndromes, while IGF-1R defects may cause growth retardation. Both signaling pathways are also implicated in cancer development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.