Thermo Fisher Scientific INSR alpha Monoclonal Antibody (83-7)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

2-4 µg/mL

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

-

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Human, Sheep, Pig, Rabbit

Published species

Human, Mouse, Yeast

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

83-7

Immunogen

IM-9 lymphocytes followed by purified insulin receptor.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536350

Product Specific Information

This antibody is specific for IR and shows no cross-reactivity with insulin-like growth factor (IGF)-receptors. The epitope for this monoclonal antibody is conformational and is located in exon 3.

Staining of formalin-fixed, paraffin tissues requires digestion of tissue sections with pepsin at 1mg/mL in Tris-HCl, pH 2.0, for 15 min at room temperature or 10 min at 37ºC. Recommended positive controls include IM-9 lymphocytes, placenta, or breast carcinoma.

Target Information

Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.