Thermo Fisher Scientific INSR alpha Monoclonal Antibody (83-14)

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Biotin Conjugated Version (MA5-13756)
• Request Custom Conjugation

Product Specific Information

This antibody reacts with an epitope at amino acids 469–592 (exon 7/8). It primarily reacts with human, but also weakly with cow, pig, and sheep.
This antibody can inhibit insulin binding (~80%) and can be used in a Tyrosine Kinase assay (antibody-mediated capture on microtitre plates).
Without BSA, it can act as an insulin-like agonist and function as both capture and detection antibody in a sandwich ELISA.

Target Information

Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through phosphorylation cascades.
Insulin/IGF-1 binding to the extracellular domain induces autophosphorylation of downstream targets.
These receptors differ in ligand specificity and intracellular signaling domains, leading to distinct functional outcomes.
Defects in insulin receptor (IR) are linked to insulin resistance syndromes, while IGF-1R defects may cause growth retardation. Both pathways are also implicated in cancer development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.