Thermo Fisher Scientific INSR alpha Monoclonal Antibody (83-14)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

2 µg / mg lysate

-

Functional Assay (Functional)

Assay-dependent

-

Inhibition Assays (IA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Sheep, Pig

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

83-14

Immunogen

IM-9 lymphocytes followed by purified insulin receptor.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536349

Product Specific Information

This antibody reacts with an epitope at aa 469-592 (exon 7/8). It primarily reacts with human, but also reacts very weakly with cow, pig and sheep.

This antibody can inhibit insulin binding (~80%). It can also be used in a Tyrosine Kinase assay (Ab-mediated capture on microtitre plates).

Without BSA, this antibody can be used as an insulin-like agonist. Without BSA, it can also be used as both a capture and detection antibody in a sandwich ELISA.

Target Information

Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.