Thermo Fisher Scientific Perforin Monoclonal Antibody (eBioBOR21), eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
The eBioBOR21 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is a cytolytic mediator present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. It plays a crucial role in immune defense against tumors and viral infections through target cell lysis.

Applications Tested:
The eBioBOR21 antibody has been tested for use in immunoblotting (WB) of human PBMCs at 1–5 µg/mL. It is recommended to titrate the antibody for optimal performance in specific assays.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Perforin is a major cytolytic protein stored in and released by cytoplasmic granules of cytotoxic lymphocytes. It is a pore-forming protein with a mechanism similar to complement component C9. Perforin consists of 555 amino acids with a 21-amino-acid signal peptide and has a molecular weight of approximately 70–75 kDa. It is specifically expressed in killer cell lines, not in helper T lymphocytes or other tumor cells.

Defects in the perforin gene are associated with familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.