Thermo Fisher Scientific Perforin Monoclonal Antibody (dG9 (delta G9)), eBioscience
인간 및 돼지 perforin 단백질을 인식하는 마우스 단클론 항체입니다. 세포내 염색, 유세포분석, 면역조직화학, 면역침전 등에 사용됩니다. 0.5 mg/mL 농도의 액상형으로, PBS(pH 7.2) 내 0.09% sodium azide를 포함합니다. 종양 및 바이러스 감염 관련 면역반응 연구에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent | 2 publications |
| Immunocytochemistry (ICC/IF) | - | 2 publications |
| Flow Cytometry (Flow) | - | 11 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Pig |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | dG9 (delta G9) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468677 |
Available Formats:
- APC (Cat. 17-9994-42)
- eFluor 450 (Cat. 48-9994-42)
- FITC (Cat. 11-9994-42)
- PE (Cat. 12-9994-42)
- PE-Cyanine7 (Cat. 25-9994-42)
- PerCP-eFluor 710 (Cat. 46-9994-42)
- Custom conjugation available upon request
Product Specific Information
Description:
The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. It plays a key role in immune responses against tumors and viral infections.
Applications Reported:
Reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. Fluorochrome-conjugated dG9 (delta G9) is recommended for intracellular flow cytometry.
Applications Tested:
Tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. Recommended usage is ≤0.25 µg per test (100 µL final volume). Optimal titration is advised for specific assays.
Purity and Quality:
- Purity: >90% (SDS-PAGE)
- Aggregation: <10% (HPLC)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Perforin is a major cytolytic protein stored in cytoplasmic granules of cytotoxic lymphocytes. It mediates immune defense against tumors and viral infections. The human perforin protein consists of 555 amino acids with a 21 amino acid signal peptide and has a molecular weight of 70–75 kDa. It forms transmembrane pores similar to complement component C9. Expression is restricted to killer cell lines and absent in helper T lymphocytes or other tumor cells. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare autosomal recessive disorder. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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