Thermo Fisher Scientific Phospho-SMAD1/SMAD5 (Ser463, Ser465) Monoclonal Antibody (E.239.4)
SMAD1/SMAD5의 Ser463/465 인산화 상태를 검출하는 Thermo Fisher의 단클론 항체로, WB, ICC/IF, Flow Cytometry 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성을 가지며 비교반응성이 없습니다. 액상 형태로 -20°C 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:800 | View publication |
| Flow Cytometry (Flow) | 1:400–1:1,600 | View publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | E.239.4 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pSer463/465 of human Smad5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 148 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_11000599 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other Smad-related proteins.
Target Information
The Smad family of proteins function in the transmission of extracellular signals in the TGF-beta signaling pathway. Binding of TGF-beta superfamily ligands to extracellular receptors triggers phosphorylation of Smad2 at a Serine-Serine-Methionine-Serine (SSMS) motif at its C-terminus. Phosphorylated Smad2 forms a complex with Smad4, which accumulates in the nucleus to regulate gene expression.
In mammals, eight Smad proteins have been identified and classified into:
- Receptor-activated Smads (R-Smads): Smad1, Smad2, Smad3, Smad5, Smad8
- Common mediator Smads (Co-Smads): Smad4
- Inhibitory Smads (I-Smads): Smad6, Smad7
R-Smads are phosphorylated by activated type I receptors on their C-terminal Ser-Ser-X-Ser (SSXS) motif. Smad2 and Smad3 respond to TGF-beta and activin, while Smad1, Smad5, and Smad8 respond to BMP (Bone Morphogenetic Protein). Phosphorylation enables R-Smad binding to Smad4 and nuclear translocation for gene regulation. I-Smads inhibit these interactions by binding to type I receptors or Smad4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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