Invitrogen™

PureLink™ Quick Plasmid Miniprep Kit

The PureLink™ Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in 30-45 minutes,자세히 알아보기

The PureLink™ Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in 30-45 minutes, using silica membrane columns. Features of the PureLink™ Quick Plasmid Miniprep Kit:

• Fast—ready-to-use plasmid DNA typically in 30-45 minutes
• Flexible—formats work with vacuum and centrifugation platforms

Reliable and cost-effective isolation of plasmid DNA
The PureLink™ Quick Plasmid Miniprep Kit allows isolation of high-quality plasmid DNA from 1–5 mL cultures in 30-45 minutes, using the PureLink™ spin columns. These columns use a unique silica membrane to achieve high yields of up to 40 μg of sequencing-grade plasmid DNA. Cells are lysed using an alkaline/SDS procedure and the lysate is applied to a silica membrane column that selectively binds plasmid DNA. Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer. The eluted DNA is suitable for all routine downstream applications.

Compatible with flexible formats
The PureLink™ Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA. Typically, DNA isolated using the PureLink™ Quick Plasmid Miniprep Kit has an A260/A280 of >1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.

Isolated DNA can be used for a variety of downstream applications
Plasmid DNA purified using PureLink™ Quick Plasmid Miniprep Kit is suitable for all routine downstream applications, including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR.

사양

농도20 mg/mL

컬럼 유형Spin Column

형식Spin Column

Isolation TechnologySilica Spin Column

샘플 종류Bacterial Culture

최종 제품 유형Plasmid DNA

용도(애플리케이션)Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation

고처리량 호환성Not High-throughput Compatible (Manual)

반응 수50 Preps

프렙 스케일< 100 μg (Small-Scale) Plasmid DNA

제품라인PureLink™

제품 유형Quick Plasmid MiniPrep Kit

수량50 Preps

배송 조건Room Temperature

타겟Plasmid DNA

테스트 시간30 to 45 min.

Unit SizeEach