Invitrogen™

TA Cloning™ Kit, Dual Promoter, with pCR™II Vector, without competent cells

The TA Cloning™ Kit Dual Promoter with pCR™II vector provides a quick, one-step cloning strategy for directly inserting a Taq-amplified자세히 알아보기

The TA Cloning™ Kit Dual Promoter with pCR™II vector provides a quick, one-step cloning strategy for directly inserting a Taq_-amplified PCR product into a plasmid vector. The T7 and Sp6 promoters of the pCR™II vector allow _in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCR™II cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

Features of the TA Cloning™ Kit Dual Promoter:
• Fast & convenient—15-minute, room-temperature ligation
• Efficient—blue/white screening and >80% clones with correct insert
• Flexible—choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free—eliminates any enzymatic modifications of the PCR product
• Streamlined—does not require the use of PCR primers that contain restriction sites

The pCR™II vector provides:
• 3`-T overhangs for direct ligation of Taq_-amplified PCR products
• T7 and Sp6 promoters for _in vitro RNA transcription and sequencing
• Versatile polylinker with flanking _Eco_R I sites for easy excision of inserts
• M13 forward and reverse primer sites for sequencing

How TA Cloning™ Works
Taq polymerase has a non-template-dependent activity that adds a single deoxyadenosine (A) to the 3ends of PCR products. The linearized vector supplied in this kit has single 3 deoxythymidine (T) residues. This allows PCR inserts to ligate efficiently with the vector.

Kit Configurations
The TA Cloning™ Kit is offered in a variety of configurations: with One Shot™ INVFChemically Competent _E. coli_ (K2050-01 and K2050-40), One Shot™ TOP10F Chemically Competent E. coli (K2060-01 and K2060-40), and without competent cells (K2750-20 and K2750-40) in 20- and 40-reaction kit sizes.

사양

박테리아 함량TOP10

박테리아 또는 효모 균주Not Included

클로닝 방법TA Cloning

형식Kit

용도(애플리케이션)PCR Cloning

반응 수20 Reactions

제품라인TA Cloning™

제품 유형Cloning Kit

프로모터T7, SP6

수량20 reactions

벡터pCRII

Unit SizeEach