Thermo Fisher Scientific CD178 (Fas Ligand) Monoclonal Antibody (NOK-1), Functional Grade, eBioscience
인간 Fas Ligand(CD178)에 특이적인 NOK-1 단클론 항체로, Flow Cytometry 및 기능적 중화 실험에 적합합니다. 1 mg/mL 농도의 액상 형태이며, 4°C에서 보관합니다. Fas/FasL 매개 세포사멸 연구 및 면역반응 조절 기전 분석에 활용됩니다.
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Thermo Fisher Scientific CD178 (Fas Ligand) Monoclonal Antibody (NOK-1), Functional Grade, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 1 µg/test | View 4 publications |
| Neutralization (Neu) | Assay-Dependent | - |
| Functional Assay | Assay-Dependent | - |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NOK-1 |
| Conjugate | Functional Grade |
| Additional Formats | APC, PE, Request custom conjugation |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2866384 |
Product Specific Information
Description:
The NOK-1 monoclonal antibody reacts with human Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family expressed by neutrophils, monocytes, activated T cells, and NK cells. Its interaction with Fas receptor induces Fas-mediated lymphocyte killing. FasL can be cleaved by MMPs to form a soluble 26 kDa protein; thus, MMP inhibitors are recommended for optimal surface detection.
Applications Reported:
- Flow cytometric analysis
- Blocking of FasL-mediated killing in functional assays
Applications Tested:
Validated for flow cytometric analysis of normal human peripheral blood cells. Recommended ≤1 µg per test (100 µL final volume). Cell number can range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.
Storage and Handling:
Use in a sterile environment.
Filtration:
0.2 µm post-manufacturing filtered.
Purity:
90%, determined by SDS-PAGE.
Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).
Aggregation:
<10%, determined by HPLC.
Target Information
CD178 (Fas ligand, FasL) is a type-II membrane protein with its N-terminus in the cytoplasm and C-terminus extracellular. Its receptor, FasR, is a type-I membrane protein of the TNF/NGF receptor family. CD178 binding to FasR triggers apoptosis via receptor oligomerization.
CD178 exists in membrane-bound (45 kDa) and soluble (26 kDa) forms, cleaved by metalloproteinases. It is expressed in activated T lymphocytes, NK cells, and immune-privileged tissues (testis, eye), as well as thymus, liver, ovary, lung, heart, and kidney. CD178-mediated apoptosis is involved in antiviral immune responses and immune regulation. Dysregulation has been linked to atherosclerosis, hypertension, AIDS, hepatitis, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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