Thermo Fisher Scientific CD281 (TLR1) Monoclonal Antibody (GD2.F4), Functional Grade, eBioscience

Thermo Fisher Scientific CD281 (TLR1) Monoclonal Antibody (GD2.F4), Functional Grade, eBioscience

Applications and Tested Dilutions

Product Specifications

Product Specific Information

The GD2.F4 antibody reacts with human Toll-like receptor 1 (TLR1). TLR1 is expressed at low levels on peripheral blood monocytes and even lower on dendritic cells, with high donor variability. eBioscience validated GD2.F4 mAb by flow cytometry using cells from healthy donors, showing similar results to the reference antibody (low TLR1 expression on PBMCs, <1/4 log shift).

Although the ligand and exact function of TLR1 are not fully defined, it is suggested that TLR1 cooperates with TLR2 in response to certain antagonists. Despite low surface expression, TLRs are efficient signal transducers in monocytes and dendritic cells. The Toll family includes at least ten members in humans, characterized by leucine-rich repeats extracellularly and an IL-1 receptor-like cytoplasmic domain. TLR2 and TLR4 are key pattern recognition receptors involved in innate immunity and inflammation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on normal human peripheral blood cells at ≤1 µg/test (100 µL final volume). Cell number should be optimized (10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.

Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

TLR1 is a member of the Toll-like receptor family, essential for pathogen recognition and activation of innate immunity. TLRs are conserved from Drosophila to humans, recognizing pathogen-associated molecular patterns (PAMPs) and mediating cytokine production for effective immune responses. TLR1 interacts with TLR2, enhancing NF-κB activation in response to synthetic lipopeptides and recognizing tri-acylated lipoproteins. Alternative splicing and polyadenylation lead to different transcript variants.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.