Thermo Fisher Scientific Zap-70 Monoclonal Antibody (1E7.2), PE, eBioscience

Thermo Fisher Scientific Zap-70 Monoclonal Antibody (1E7.2), PE, eBioscience™

Applications

• Flow Cytometry (Flow): Tested at 1 µg/test
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Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 647
• FITC
• Request custom conjugation

Product Specific Information

Description:
The 1E7.2 antibody reacts with human and mouse ZAP-70, a cytosolic protein tyrosine kinase (PTK) of the Syk family. It is expressed in T and NK cells and is essential for TCR signaling and development. ZAP-70 binds to phosphorylated ITAMs in the TCR complex and becomes phosphorylated via autophosphorylation and transphosphorylation by Lck. Tyrosine phosphorylation increases kinase activity and triggers downstream signaling. Mutations in ZAP-70 are associated with Severe Combined Immunodeficiency Syndrome (SCID). The antibody was generated against a KLH-peptide sequence corresponding to human ZAP-70 amino acid residues 282–307.

ZAP-70 expression correlates with mutated IgVH in B-chronic lymphocytic leukemia (B-CLL) and may serve as a prognostic marker measurable by intracellular flow cytometry.

Applications Reported:
Reported for intracellular flow cytometric analysis.

Applications Tested:
Tested by intracellular flow cytometric analysis of mouse thymocytes and human Jurkat cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol.
Refer to BestProtocols®: Protocol B — One-step protocol for nuclear intracellular proteins.
Use ≤1 µg per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Excitation / Emission / Laser:
488–561 nm / 578 nm / Blue, Green, Yellow-Green lasers.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

ZAP-70 is a cytosolic protein tyrosine kinase of the Syk family, expressed exclusively in T-cells and NK cells. It is required for T-cell receptor activation. Upon TCR engagement, ZAP-70 is phosphorylated on tyrosines 292, 315, and 319. Phosphorylation at 315 (Vav binding site) and 319 (Lck binding site) enhances signaling, while phosphorylation at 292 terminates activation and attenuates signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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