Thermo Fisher Scientific Zap-70 Monoclonal Antibody (1E7.2), PE, eBioscience
Human 및 Mouse ZAP-70 단백질을 검출하는 PE 결합 단일클론 항체. T 및 NK 세포에서 TCR 신호전달 연구에 적합. Flow cytometry용으로 최적화되어 있으며 0.2 mg/mL 농도의 액상 형태로 제공. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific Zap-70 Monoclonal Antibody (1E7.2), PE, eBioscience™
Applications
- Flow Cytometry (Flow): Tested at 1 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E7.2 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466141 |
Additional Formats
Product Specific Information
Description:
The 1E7.2 antibody reacts with human and mouse ZAP-70, a cytosolic protein tyrosine kinase (PTK) of the Syk family. It is expressed in T and NK cells and is essential for TCR signaling and development. ZAP-70 binds to phosphorylated ITAMs in the TCR complex and becomes phosphorylated via autophosphorylation and transphosphorylation by Lck. Tyrosine phosphorylation increases kinase activity and triggers downstream signaling. Mutations in ZAP-70 are associated with Severe Combined Immunodeficiency Syndrome (SCID). The antibody was generated against a KLH-peptide sequence corresponding to human ZAP-70 amino acid residues 282–307.
ZAP-70 expression correlates with mutated IgVH in B-chronic lymphocytic leukemia (B-CLL) and may serve as a prognostic marker measurable by intracellular flow cytometry.
Applications Reported:
Reported for intracellular flow cytometric analysis.
Applications Tested:
Tested by intracellular flow cytometric analysis of mouse thymocytes and human Jurkat cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol.
Refer to BestProtocols®: Protocol B — One-step protocol for nuclear intracellular proteins.
Use ≤1 µg per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Titrate for optimal performance.
Excitation / Emission / Laser:
488–561 nm / 578 nm / Blue, Green, Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase of the Syk family, expressed exclusively in T-cells and NK cells. It is required for T-cell receptor activation. Upon TCR engagement, ZAP-70 is phosphorylated on tyrosines 292, 315, and 319. Phosphorylation at 315 (Vav binding site) and 319 (Lck binding site) enhances signaling, while phosphorylation at 292 terminates activation and attenuates signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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