Thermo Fisher Scientific TCL1 Monoclonal Antibody (eBio1-21 (1-21)), PE, eBioscience
인간 TCL1 단백질을 표적으로 하는 eBio1-21 (1-21) 클론의 단클론 항체로, PE 형광체가 결합되어 있습니다. 유세포 분석용으로 사전 희석 및 검증되었으며, 4°C 암소 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific TCL1 Monoclonal Antibody (eBio1-21 (1-21)), PE, eBioscience™
Applications
- Flow Cytometry (Intracellular staining)
Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio1-21 (1-21) |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10853340 |
Available Formats:
- Unconjugated
- APC
- eFluor 450
- PE-Cyanine7
- Custom conjugation available upon request
Product Specific Information
The eBio1-21 antibody reacts with human T cell leukemia/lymphoma 1 (TCL1), a 14 kDa proto-oncogene involved in intracellular regulation of T cell signaling. TCL1 is located at the 14q32.1 chromosome breakpoint region in T-cell prolymphocytic leukemia (T-PLL) and is overexpressed due to chromosomal rearrangements that juxtapose it with T-cell receptor promoter elements.
TCL1 interacts with the pleckstrin homology domain of Akt family proteins, enhancing Akt dimerization and activity, which increases phosphorylation of key signaling substrates such as Ikk complex, mTOR, BAD, p70S6 kinase, FOXO transcription factors, and GSK3β. These substrates regulate cell differentiation, growth, survival, and metabolism.
TCL1 is normally expressed in specific subsets of thymic precursors, plasmacytoid dendritic cells, activated T cells, and B cells up to the germinal center stage. Aberrant expression is associated with T cell malignancies and chronic lymphocytic leukemia (CLL).
Applications Reported:
- Intracellular staining followed by flow cytometric analysis
Applications Tested:
- Pre-titrated and validated for intracellular staining of Daudi cells
- Recommended use: 5 µL (0.125 µg) per test in 100 µL final volume
- Suitable for 10⁵–10⁸ cells/test
Optical Properties:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Overexpression of TCL1 in humans is linked to mature T cell leukemia through chromosomal rearrangements that bring TCL1 near TCR regulatory elements. In normal T cells, TCL1 is expressed in CD4-/CD8- cells but not in later differentiation stages. TCL1 acts as a coactivator of the cell survival kinase AKT.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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