Thermo Fisher Scientific Syk Monoclonal Antibody (4D10.1), PE, eBioscience
인간 Syk 단백질을 인식하는 4D10.1 단클론 항체로, PE 형광 표지되어 Flow Cytometry용으로 최적화됨. B세포 및 면역 수용체 신호전달 연구에 적합. 5 µL/test로 사전 희석 및 검증 완료. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 12-6696-42
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Syk Monoclonal Antibody (4D10.1), PE, eBioscience
Thermo Fisher Scientific Syk Monoclonal Antibody (4D10.1), PE, eBioscience™
Applications
- Intracellular staining
- Flow Cytometric analysis
Tested Dilution
- 5 µL (0.125 µg)/test
Publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4D10.1 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572650 |
Additional Formats
Product Specific Information
Description:
The 4D10.1 monoclonal antibody recognizes human Syk, a 72-kDa non-receptor protein tyrosine kinase of the Syk/ZAP-70 family. Syk is highly expressed in B cells, with lower levels in thymocytes, mast cells, and platelets, and can be detected in fibroblasts, epithelial cells, hepatocytes, endothelial, and neuronal cells.
Syk is crucial in B and T cell antigen receptor signaling, activated by phosphorylation upon ITAM recruitment, and phosphorylates downstream proteins such as PLCγ and BLNK/SLP-65. Abnormal Syk expression is associated with tumor cell migration and invasion.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis.
Applications Tested:
- Pre-titrated and validated for intracellular staining and flow cytometry of human peripheral blood cells using Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
- Recommended use: 5 µL (0.125 µg) per test (100 µL final volume).
- Applicable for 10⁵–10⁸ cells/test.
Excitation / Emission / Laser:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
Filtration:
- 0.2 µm post-manufacturing filtered
Target Information
Spleen tyrosine kinase (SYK) and ZAP70 are cytoplasmic tyrosine kinases involved in allergic and autoimmune disease pathways and may play roles in cancer.
Upon activation by BCR or FcεRI, Syk translocates to the plasma membrane and phosphorylates adaptor proteins, initiating downstream signaling such as calcium mobilization, cytoskeleton remodeling, and gene transcription.
Syk binds via its SH2 domains to phosphorylated ITAM motifs, and is further activated by Src-family kinase phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.