Thermo Fisher Scientific Perforin Monoclonal Antibody (eBioOMAK-D), eBioscience
마우스 퍼포린을 인식하는 단클론 항체로, CTL 및 NK 세포의 세포독성 단백질 분석에 적합합니다. 웨스턴 블롯 및 유세포 분석에 사용 가능하며, 높은 순도(>90%)와 낮은 응집도(<10%)를 보유합니다. IL-2 자극 시 퍼포린 발현 연구에 유용합니다.
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Thermo Fisher Scientific Perforin Monoclonal Antibody (eBioOMAK-D), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | - | View 26 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioOMAK-D |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865375 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugated Formats
Product Specific Information
The eBioOMAK-D antibody reacts with mouse perforin (pore-forming protein, pfp, Prf). Perforin is a cytolytic mediator found in cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells, playing a crucial role in immune responses against tumors and viral infections.
By immunoblotting, eBioOMAK-D recognizes a ~70 kDa band in lysates of CTLL-2 mouse cytotoxic cells and IL-2 stimulated splenocytes, but not in unstimulated ones. Flow cytometric analysis shows increased staining upon IL-2 or anti-CD3/CD28 stimulation. Perforin knockout splenocytes do not stain or show detectable protein by western blotting.
In IL-2 stimulated splenocytes:
- NK cells (CD49b+) contain perforin.
- CD8 cells show variable expression depending on culture conditions.
- Anti-CD3/CD28 stimulation increases perforin expression in both NK and CD8 cells.
eBioOMAK-D is also cross-reactive to human perforin and co-stains CD56+ PBMC cells.
Granzyme B and perforin expression may not correlate; Granzyme B increases earlier and more dramatically (>10,000-fold mRNA increase) compared to perforin (10–100-fold).
For intracellular staining and flow cytometry, use the Foxp3 buffer system (Product #00-5523) for optimal results.
Applications Reported: Immunoblotting (WB)
Applications Tested: Immunoblotting of CTLL-2 cell lysates ≤ 2 µg/mL
Target Information
Perforin is a major cytolytic protein stored in cytoplasmic granules of cytotoxic lymphocytes. It plays an essential role in immune defense against tumors and viruses.
- Molecular Weight: 70–75 kDa
- Length: 555 amino acids (includes 21-aa signal peptide)
- Mechanism: Pore-forming, similar to complement component C9
- Expression: Found in killer cell lines, absent in helper T cells and other tumor cells
- Clinical Relevance: Mutations cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare autosomal recessive disorder.
- Alternative splicing produces multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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