Thermo Fisher Scientific Estrogen Receptor beta Monoclonal Antibody (MC10), eBioscience
인간 Estrogen Receptor beta(ERβ)를 인식하는 MC10 단클론 항체로, 다양한 ERβ 형태 및 스플라이스 변형체를 검출 가능. WB, IHC, ICC, ELISA, IP 등 다중 응용에 적합. 고순도(>90%) 및 낮은 응집도(<10%)를 가진 액상 항체로 연구용에 최적화.
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Thermo Fisher Scientific Estrogen Receptor beta Monoclonal Antibody (MC10), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | View 2 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 30 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | View 1 publication |
| ELISA | Assay-Dependent | - |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MC10 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572942 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The MC10 monoclonal antibody recognizes all forms of human estrogen receptor (ER) beta. ER beta is a member of the nuclear receptor superfamily, functioning as a ligand-dependent transcription factor. Upon estrogen binding, ER beta dissociates from chaperone proteins and dimerizes with ER beta or ER alpha. Unlike ER alpha, ER beta signaling is considered anti-proliferative and pro-apoptotic.
MC10 clone detects full-length ER beta and all known splice variants.
Applications Reported:
Immunoprecipitation, Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, microscopy, ELISA, and immunocytochemistry.
Applications Tested:
Validated for IHC on formalin-fixed paraffin-embedded human tissue using low or high pH antigen retrieval at ≤30 µg/mL. Antibody titration is recommended for optimal performance.
Target Information
Estrogen Receptor beta (ERβ, NR3A2) is approximately 55 kDa, with isoforms ranging from 36–59 kDa. Initially cloned from human testis, ERβ belongs to the steroid/thyroid hormone receptor superfamily. Upon estrogen binding, ERβ undergoes conformational change, dimerizes, and binds to estrogen response elements (EREs) in DNA, regulating transcription via coactivator or corepressor recruitment.
Two receptor forms exist, ERα (ESR1) and ERβ (ESR2), both with similar estrogen-binding affinities. Certain ERβ isoforms inhibit ERα activity in reproductive tissues. ERβ is broadly expressed and acts as a tumor suppressor, implicated in prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain disease, and cardiovascular disease risk.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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