Thermo Fisher Scientific ASAP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,500

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:1,000

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, conjugated to KLH, corresponding to residues 1128-1147 of mouse Centaurin beta 4. The peptide sequence is identical in bovine ASAP1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ASAP1,uniProtId:Q9QWY8-1,ncbiNodeId:10090,antigenRange:1128-1147,antigenLength:1147,antigenImageFileName:PA1-25512_ASAP1_Q9QWY8-1_Rabbit.svg,antigenImageFileNamePDP:PA1-25512_ASAP1_Q9QWY8-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

11.6 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2058880

Product Specific Information

Recommended positive controls: rat brain, mouse brain, NIH3T3.

PA1-25512 is expected to cross react with bovine due to sequence homology.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ASAP1, also known as Development and differentiation enhancing factor 1, is a protein with two ANK repeats, an Arf-GAP domain, a PH domain and a SH3 domain. ASAP1 is a homodimer known to interact with SRC and CRK and its activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2). It possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. It may coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2 and may also function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. It is widely expressed in most of the tissues. ASAP1 is a protooncogene involved in invasive breast cancer and in other types of invasive and malignant tumors, such as uveal melanomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.