Thermo Fisher Scientific ASAP1 Polyclonal Antibody
Rabbit polyclonal antibody against ASAP1, validated for WB, IHC(P), and ICC/IF. Recognizes mouse and bovine ASAP1. Suitable for detecting ASAP1 in brain tissues and fibroblast differentiation studies. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,500 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to residues 1128–1147 of mouse Centaurin beta 4. The peptide sequence is identical in bovine ASAP1. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 11.6 mg/mL |
| Storage Conditions | Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2058880 |
Product Specific Information
- Recommended positive controls: rat brain, mouse brain, NIH3T3.
- Expected to cross-react with bovine due to sequence homology.
- Store product as a concentrated solution.
- Centrifuge briefly before opening the vial.
Target Information
ASAP1 (Development and differentiation enhancing factor 1) is a protein containing two ANK repeats, an Arf-GAP domain, a PH domain, and a SH3 domain. It forms a homodimer that interacts with SRC and CRK, with activity stimulated by phosphatidylinositol 4,5-biphosphate (PIP2).
ASAP1 functions as a phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein for ARF1 and ARF5, with lesser activity toward ARF6. It may coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to SRC and PIP2.
Additionally, it may act as a signal transduction protein involved in fibroblast differentiation into adipocytes and other cell types. It is widely expressed in most tissues and is considered a proto-oncogene implicated in invasive breast cancer and other malignant tumors such as uveal melanomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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