Merck CRISPR GUS GAPDH Reporter Control for Monocots

CRISPR GUS GAPDH Reporter Control for Monocots

Quality Level

200

재조합

expressed in E. coli

포장

vial of 50 μL

농도

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

application(s)

CRISPR

selection

kanamycin

배송 상태

dry ice

저장 온도

−20°C

All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids with GUS Reporter

CRISPR Plant Cas9 products are intended for Agrobacterium-mediated plant transformation. The products are based on the type IIA CRISPR-Cas9 derived from S_treptococcus pyogenes_. The native Cas9 coding sequence is codon optimized for expression in monocots and dicots, respectively. The monocot Cas9 constructs contain a monocot U6 promoter for sgRNA expression, and the dicot Cas9 constructs contain a dicot U6 promoter.

Arabidopsis seedlings were germinated in 6 well tissue culture plates. The seedlings were infected with Agrobacterium which had the CRISPR plasmids with a GUS reporter. After 3-4 days of transfection the GUS expression was detected. b-glucuronidase (GUS) is an enzyme that hydrolyzes colorless glucuronides to yield colored product