Merck CRISPR GUS GAPDH Reporter Control for Monocots

CRISPR GUS GAPDH Reporter Control for Monocots

All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids with GUS Reporter.

CRISPR Plant Cas9 products are intended for Agrobacterium-mediated plant transformation. These products are based on the type IIA CRISPR-Cas9 system derived from Streptococcus pyogenes.
The native Cas9 coding sequence is codon optimized for expression in monocots and dicots, respectively.

• Monocot Cas9 constructs: contain a monocot U6 promoter for sgRNA expression
• Dicot Cas9 constructs: contain a dicot U6 promoter

실험 예시

Arabidopsis seedlings were germinated in 6-well tissue culture plates and infected with Agrobacterium carrying the CRISPR plasmids with a GUS reporter. After 3–4 days of transfection, GUS expression was detected.
β-glucuronidase (GUS) is an enzyme that hydrolyzes colorless glucuronides to yield a colored product.

제품 사양