Merck Cas9 Hygromycin Lenti Plasmid

Cas9 Hygromycin Lenti Plasmid

재조합

expressed in E. coli

form

liquid

포장

vial of 50 μL

농도

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

application(s)

CRISPR

selection

blasticidin

배송 상태

dry ice

저장 온도

−20°C

This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and a hygromycin resistance cassette linked by a 2A peptide (EF1a-Cas9-2A-Hygro) allowing for easy visualization of successful transfection or transduction. Use Sigma′s lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigma′s Cas9-Hygro lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors.

To order gRNA in any format click here