Merck eSpCas9-Blasticidin Lenti Plasmid

eSpCas9-Blasticidin Lenti Plasmid

Quality Level

200

재조합

expressed in E. coli

포장

vial of 50 μL

농도

20 ng/μL in TE buffer; DNA (1μg of Plasmid DNA)

selection

ampicillin

배송 상태

dry ice

저장 온도

−20°C

This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of eSpCas9 and a blasticidin resistance cassette linked by a 2A peptide (EF1a-eSpCas9-2A-Blasticidin) allowing for easy selection following successful transfection or transduction. Use Sigma′s lentiviral eSpCas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing eSpCas9 for CRISPR based genome editing. Sigma′s lenti-eSpCas9 plasmid is one part of a two part CRISPR system with individual eSpCas9 and gRNA expression vectors.

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