Takara PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time): Eliminate genomic DNA contamination

Overview

• Genomic DNA contamination elimination in only 2 minutes

• Fast cDNA synthesis—reverse transcription in just 15 minutes

• Suitable for use with routine and challenging RNA templates alike

• Full-length cDNA synthesis up to 12 kb

More Information

Applications

• Elimination of genomic DNA contamination

• cDNA synthesis for real-time RT-PCR (qPCR)

• Two-step real-time RT-PCR

Choose the right PrimeScript kit for real-time RT-PCR:

If you want to....	Use this....
Make cDNA using a master mix that includes RTase and primers	PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A and RR036B)
Make cDNA using a kit that provides RTase and primers as separate components	PrimeScript RT Reagent Kit (Perfect Real Time) (Cat. # RR037A and RR037B)
Avoid genomic DNA contamination during cDNA synthesis and RT-qPCR	PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Cat. # RR047A and RR047B)
Perform 1-step real-time RT-PCR with TB Green	One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Cat. # RR086A and RR086B)
Prepare cDNA for RT-qPCR directly from fewer than 1,000 cells	CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2 (Cat. # 3734)

Product citations

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Yang, Y. et al. Expression of the laccase gene from a white rot fungus in Pichia pastoris can enhance the resistance of this yeast to H2O2-mediated oxidative stress by stimulating the glutathione-based antioxidative system. Appl. Environ. Microbiol. 78, 5845–54 (2012).

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Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.