Takara SMARTer Mouse BCR IgG H/K/L Profiling Kit—capturing complete V(D)J variable regions of BCR transcripts

SMARTer Mouse BCR IgG H/K/L Profiling Kit

The SMARTer Mouse BCR IgG H/K/L Profiling Kit pairs 5′ RACE with NGS technology to provide a sensitive, accurate, and optimized approach to BCR profiling.
The 5′-RACE method reduces variability and allows for priming from the constant region of BCR heavy or light chains.
This kit combines these benefits with gene-specific amplification to capture complete V(D)J variable regions of BCR transcripts and provide a highly sensitive and reproducible method for profiling B-cell repertoires.

The kit is designed to work with a range of RNA input amounts depending on the sample type, and has been shown to generate high-quality, Illumina-ready libraries from as little as 10 ng to 3 µg of total RNA from spleen, lymph node, peripheral blood mononuclear cells (PBMCs), and hybridomas.

Downstream sequencing of H/K/L chains allows for accurate identification of top clonotypes and reliable assignment of isotype in a majority of cases.

Overview

• Input: 10 ng–3 µg of total RNA from spleen, lymph node, PBMCs, and hybridomas
• Sensitive and specific clonotype detection with optimized cDNA library generation
• Accurate amplification of mouse IgG subclasses and identification via sequencing in a majority of cases
• Optimized PCR pooling strategy for highly sensitive detection of different chains from the same sample
• Flexible pooling strategies accommodate different experimental requirements regarding alignment and identification of primary chain sequences

Applications

• Mouse BCR repertoire analysis (heavy and light [kappa and lambda] chains)

Additional Product Information

Please refer to the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications.
See the Kit Components List under the Documents tab for detailed component information.