Thermo Fisher Scientific POLR2A Monoclonal Antibody (OTI2B7), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

DNA-directed RNA polymerase II subunit RPB1 (POLR2A) is a DNA-dependent RNA polymerase that catalyzes transcription of DNA into RNA using ribonucleoside triphosphates as substrates.
POLR2A is the largest subunit and a catalytic component of RNA polymerase II, responsible for synthesizing mRNA precursors and various functional non-coding RNAs.

It forms the polymerase active center with the second largest subunit and is integral to the basal transcription machinery. The enzyme includes mobile elements that move relative to each other, with RPB1 forming the core structure containing the large cleft, clamp, and jaws that interact with the DNA template.

Transcription initiation involves positioning the single-stranded DNA template within the central active site cleft. A bridging helix from RPB1 promotes translocation of Pol II by acting as a ratchet mechanism during nucleotide addition.

During elongation, Pol II progresses along the template as the transcript extends. This process is regulated by phosphorylation of the C-terminal domain (CTD) of RPB1, which serves as a scaffold for factors controlling initiation, elongation, termination, and mRNA processing. Gene expression regulation depends on the balance of methylation and acetylation of CTD lysines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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