Thermo Fisher Scientific P4HTM Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,500

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-Dependent

Immunocytochemistry (ICC/IF)

• Tested Dilution: Assay-Dependent

Immunomicroscopy (IM)

• Tested Dilution: Assay-Dependent

Product Specifications

Product Specific Information

• This antibody does not react with mouse samples.
• Suggested positive control: antigen standard for PH (transient overexpression lysate), HeLa and human glioblastoma cell lysates.

Target Information

Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen.
HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction.
Low oxygen levels cause the HIF conversion to be reduced, stabilizing HIF and increasing angiogenesis. Prolyl hydroxylase 4 catalyzes the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins and may function as a cellular oxygen sensor, targeting HIF for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex under normoxic conditions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.