Thermo Fisher Scientific P4HTM Polyclonal Antibody
Thermo Fisher Scientific의 P4HTM Polyclonal Antibody는 Human 및 Rat 시료에 반응하며, HIF-alpha의 프로린 하이드록실레이스 4를 검출합니다. Western blot 및 IHC 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제된 고품질 액상 항체입니다.
- 카탈로그번호
- PA116560
- 판매단위
- pk
카탈로그
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,500
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: Assay-Dependent
Immunocytochemistry (ICC/IF)
- Tested Dilution: Assay-Dependent
Immunomicroscopy (IM)
- Tested Dilution: Assay-Dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | The epitope recognized by this antibody maps to a region between residue 450 and the C-terminus (residue 502) of human PHD4/HIF Prolyl Hydroxylase 4 (GeneID 54681) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Tris citrate/tris phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, do not freeze |
| Shipping conditions | Wet ice |
| RRID | AB_2156599 |
Product Specific Information
- This antibody does not react with mouse samples.
- Suggested positive control: antigen standard for PH (transient overexpression lysate), HeLa and human glioblastoma cell lysates.
Target Information
Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen.
HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction.
Low oxygen levels cause the HIF conversion to be reduced, stabilizing HIF and increasing angiogenesis. Prolyl hydroxylase 4 catalyzes the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins and may function as a cellular oxygen sensor, targeting HIF for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex under normoxic conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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