Thermo Fisher Scientific MECP2 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

PA1‑888 detects methyl CpG binding protein 2 (MeCP2) from human, mouse, and rat cells and tissues.
It has been successfully used in Western blot procedures, detecting an ~56 kDa MeCP2 protein from AtT20 cell extract.
The immunizing peptide corresponds to amino acid residues 469–486 of human MeCP2, a sequence completely conserved in rat and mouse.
The immunizing peptide (Cat. # PEP‑121) is available for use in neutralization and control experiments.

Target Information

MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3, and MBD4) that contain a methyl‑CpG binding domain.
It binds specifically to methylated DNA, a key modification in eukaryotic genomes crucial for mammalian development.
MECP2 represses transcription from methylated gene promoters and, unlike other MBD family members, is X‑linked and subject to X inactivation.
While dispensable in stem cells, MECP2 is essential for embryonic development.
Mutations in the MECP2 gene cause most cases of Rett syndrome, a progressive neurological developmental disorder and a common cause of mental retardation in females.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.