Thermo Fisher Scientific MECP2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C P(469) N R E E P V D S R T P V T E R V S(486) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MECP2,uniProtId:P51608-1,ncbiNodeId:9606,antigenRange:469-486,antigenLength:486,antigenImageFileName:PA1-888_MECP2_P51608-1_Rabbit.svg,antigenImageFileNamePDP:PA1-888_MECP2_P51608-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2281966

Product Specific Information

PA1-888 detects methyl CpG binding protein 2 (MeCP2) from human, mouse and rat cells and tissues.

PA1-888 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~56 kDa protein representing MeCP2 from AtT20 cell extract.

PA1-888 immunizing peptide corresponds to amino acid residues 469-486 from human MeCP2. This sequence is completely conserved in rat and mouse MeCP2. PA1-888 immunizing peptide (Cat. # PEP-121) is available for use in neutralization and control experiments.

Target Information

MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3 and MBD4) that have a methyl-CpG binding domain. MECP2 is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. MECP2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.