Thermo Fisher Scientific MECP2 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-887 detects methyl CpG binding protein 2 (MeCP2) from human, mouse, and rat tissues and cells.
It has been successfully used in Western blot, IHC-P, immunofluorescence, and immunoprecipitation procedures.
By Western blot, this antibody detects an ~56 kDa protein representing MeCP2 from AtT20 cell extract.
PA1-887 immunizing peptide corresponds to amino acid residues 1–15 from mouse MeCP2, which is completely conserved in human MeCP2.
The immunizing peptide (Cat. # PEP-120) is available for use in neutralization and control experiments.

Target Information

MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3, and MBD4) that contain a methyl-CpG binding domain.
MECP2 binds specifically to methylated DNA, playing a crucial role in mammalian development and transcriptional repression of methylated gene promoters.
Unlike other MBD family members, MECP2 is X-linked and subject to X inactivation.
Mutations in the MECP2 gene are the primary cause of Rett syndrome, a progressive neurological developmental disorder and a common cause of mental retardation in females.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.