Thermo Fisher Scientific MBD1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C R(593) S K D L K K P G A R K Q(605) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MBD1,uniProtId:Q9UIS9-1,ncbiNodeId:9606,antigenRange:593-605,antigenLength:605,antigenImageFileName:PA1-889_MBD1_Q9UIS9-1_Rabbit.svg,antigenImageFileNamePDP:PA1-889_MBD1_Q9UIS9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2139592

Product Specific Information

PA1-889 detects human methyl CpG binding domain protein 1 (MBD1) recombinant protein.

PA1-889 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~83 kDa protein representing recombinant human MBD1.

PA1-889 immunizing peptide corresponds to amino acid residues 593-605 from human MBD1 protein. PA1-889 immunizing peptide (Cat. # PEP-130) is available for use in neutralization and control experiments.

Target Information

Methyl-CpG-binding domain protein 1 (MBD1) is a member of a family of mammalian proteins that specifically bind methylated regions of DNA. It is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). MBD1 acts as a transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.