Thermo Fisher Scientific SIN3A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

ChIP assay (ChIP)

1-3 µL

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) K R R L D D Q E S P V Y A A Q Q R R(19) of mouse mSin3A. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SIN3A,uniProtId:Q60520-2,ncbiNodeId:10090,antigenRange:1-19,antigenLength:1274,antigenImageFileName:PA1-870_SIN3A_Q60520-2_Rabbit.svg,antigenImageFileNamePDP:PA1-870_SIN3A_Q60520-2_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2187625

Product Specific Information

PA1-870 detects mSin3A from human and mouse tissues and cells.

PA1-870 has been successfully used in Western blot, ICC/IF ChIP, IHC-P and immunoprecipitation procedures. By Western blot, this antibody detects an ~150 kDa protein representing mSin3A from T47D cell extract.

PA1-870 immunogen is a synthetic peptide corresponding to residues M(1) K R R L D D Q E S P V Y A A Q Q R R(19) of mouse mSin3A.

Target Information

Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.