Thermo Fisher Scientific p27/Kip Polyclonal Antibody
Western blot, IHC, IP 등 다양한 응용에 적합한 p27/Kip1 항체. Human, Mouse, Rat 반응성. Rabbit Polyclonal IgG로 항원 친화 크로마토그래피 정제. 세포주기 조절 연구에 유용하며, 4°C 단기 보관 및 -20°C 장기 보관 권장.
- 카탈로그번호
- 500-2604
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the C-terminus of human p27 (UniProt ID: P46527-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human colon tissue
- Cellular location: Nucleus, Cytoplasm, Endosome
Target Information
p27 Kip1 functions as a negative regulator of G1 phase expression and is proposed to mediate TGFβ-induced G1 arrest. It is a key regulator of cell cycle progression and a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms complexes with cyclin D-CDK4, influencing assembly, stability, and activation of CCND1-CDK4. Depending on its phosphorylation state or stoichiometry, p27 Kip1 can act as either inhibitor or activator of cyclin D-CDK4 complexes. With a molecular weight of approximately 27 kDa, its degradation—triggered by CDK-dependent phosphorylation and ubiquitination by SCF complexes—is essential for the transition from quiescence to the proliferative state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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