Thermo Fisher Scientific SR Monoclonal Antibody (16H3 (16H3E8))

Applications

Tested Dilution

Publications

Western Blot (WB)

3-5 µg/mL

View 12 publications 12 publications

Immunocytochemistry (ICC/IF)

0.5-2 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

0.1-1 µg/mL

-

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Fruit fly, Human, Mouse, Rabbit, Rat, Xenopus

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

16H3 (16H3E8)

Immunogen

Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST. The 16H3 epitope has been mapped to a 40-amino acid polypeptide composed almost exclusively of arginine alternating with glutamate and aspartate

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533149

Product Specific Information

This monoclonal antibody can be used to detect a sub-set of the non-snRNP splicing factors termed SR proteins. The antibody detects SRp75, SRp55, SRp40, and SRp20 but not SRp30a or b (ASF/SF2, SC-35) proteins. 16H3 also recognizes approximately 20 distinct nuclear proteins including the U1 70K component of the U1 snRNP and both subunits of U2AF. The epitope detected by the 16H3 monoclonal antibody has been termed the ″alternating arginine domain″ and is composed almost exclusively of argnine alternating with glutamate and aspartate. 16H3 targets are predominantly nuclear, nonnucleolar, and are localized to active sites of polymerase II transcription.

Target Information

SR proteins are a highly conserved family of arginine/serine-rich, spliceosome-associated phosphoproteins essential for metazoan pre-mRNA splicing. SR proteins act early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also play a regulatory role because they can determine alternative splice site usage in vivo and in vitro. SR proteins appear to be recruited from nuclear ″speckles″, in which they are concentrated to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.