Thermo Fisher Scientific SR Monoclonal Antibody (16H3 (16H3E8))

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This monoclonal antibody detects a subset of non-snRNP splicing factors known as SR proteins. It recognizes SRp75, SRp55, SRp40, and SRp20, but not SRp30a or b (ASF/SF2, SC-35). The 16H3 antibody also identifies approximately 20 distinct nuclear proteins, including the U1 70K component of the U1 snRNP and both subunits of U2AF. The epitope, termed the “alternating arginine domain,” consists almost entirely of arginine alternating with glutamate and aspartate. 16H3 targets are primarily nuclear, non-nucleolar, and localized to active sites of RNA polymerase II transcription.

Target Information

SR proteins are a conserved family of arginine/serine-rich, spliceosome-associated phosphoproteins essential for metazoan pre-mRNA splicing. They function early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also regulate alternative splice site usage in vivo and in vitro and are recruited from nuclear “speckles” to transcription sites to coordinate transcription and pre-mRNA splicing within the nucleus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.