Thermo Fisher Scientific SR Monoclonal Antibody (16H3 (16H3E8))
SR 단백질 검출용 마우스 단클론 항체로, 다양한 종에서 반응하며 WB, ICC/IF, ELISA, IP 등 다중 응용 가능. 비결합형 액상 형태로 제공되며, 단백질 A 정제 및 0.5 mg/mL 농도. 핵 내 전사 활성 부위의 SR 단백질 인식.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 3–5 µg/mL | View 12 publications |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL | View 2 publications |
| ELISA | 0.1–1 µg/mL | - |
| Immunoprecipitation (IP) | – | View 2 publications |
| Miscellaneous PubMed (Misc) | – | View 3 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Fruit fly, Human, Mouse, Rabbit, Rat, Xenopus |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16H3 (16H3E8) |
| Immunogen | Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST. The 16H3 epitope has been mapped to a 40-amino acid polypeptide composed almost exclusively of arginine alternating with glutamate and aspartate. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 1 month. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533149 |
Product Specific Information
This monoclonal antibody detects a subset of non-snRNP splicing factors known as SR proteins. It recognizes SRp75, SRp55, SRp40, and SRp20, but not SRp30a or b (ASF/SF2, SC-35). The 16H3 antibody also identifies approximately 20 distinct nuclear proteins, including the U1 70K component of the U1 snRNP and both subunits of U2AF. The epitope, termed the “alternating arginine domain,” consists almost entirely of arginine alternating with glutamate and aspartate. 16H3 targets are primarily nuclear, non-nucleolar, and localized to active sites of RNA polymerase II transcription.
Target Information
SR proteins are a conserved family of arginine/serine-rich, spliceosome-associated phosphoproteins essential for metazoan pre-mRNA splicing. They function early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also regulate alternative splice site usage in vivo and in vitro and are recruited from nuclear “speckles” to transcription sites to coordinate transcription and pre-mRNA splicing within the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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