Thermo Fisher Scientific ZO-1 Monoclonal Antibody (ZO1-1A12), FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 14 publications 14 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1-10 µg/mL

View 20 publications 20 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.1-1.0 µg/mL

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Dog, Human

Published species

Dog, Human, Mouse, Rat, Zebrafish

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ZO1-1A12

Immunogen

Human recombinant ZO-1 fusion protein encompassing amino acids 334-634 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ZO-1,uniProtId:Q07157-1,ncbiNodeId:9606,antigenRange:334-634,antigenLength:1748,antigenImageFileName:33-9111_ZO-1_Q07157-1_House_mouse.svg,antigenImageFileNamePDP:33-9111_ZO-1_Q07157-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 594
• Alexa Fluor 647
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533148

Product Specific Information

Species reactivity includes human (Caco-2 Cell line) and dog (MDCK cell line). Based on sequence homology, reactivity with other species is likely but has not been confirmed.

This monoclonal antibody detects ZO-1alpha+ and ZO-1alpha- isoforms. The antibody is specific for ZO-1 proteins; cross-reactivity with the related ZO-2 protein has not been observed.

For this FITC conjugate the excitation (EX)/emission (EM) are 494/520 (nm).

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.