Thermo Fisher Scientific BRCA1 Monoclonal Antibody (MS13)
BRCA1 단백질을 특이적으로 인식하는 MS13 클론의 마우스 단일클론 항체입니다. Western blot, IHC, ICC/IF, IP, RIP 등 다양한 응용에 적합합니다. Human BRCA1 N-말단 영역(1–304 aa)을 인식하며, 단백질 A로 정제된 액상 형태로 제공됩니다.
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Thermo Fisher Scientific BRCA1 Monoclonal Antibody (MS13)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1.5–2.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Immunoprecipitation (IP) | 5–10 µL per 500 µg lysate |
| RNA Immunoprecipitation (RIP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MS13 |
| Immunogen | Recombinant full-length human BRCA1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536843 |
Product Specific Information
Antibody binding site (epitope) has been mapped within the N-terminal (1–304 amino acids) of Human BRCA1.
Target Information
BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein involved in maintaining genomic stability and functioning as a tumor suppressor. It forms part of the BASC complex (BRCA1-associated genome surveillance complex) with other tumor suppressors, DNA damage sensors, and signal transducers. BRCA1 interacts with RNA polymerase II and histone deacetylase complexes, playing critical roles in transcription regulation, DNA double-strand break repair, and recombination. Mutations in BRCA1 account for approximately 40% of inherited breast cancers and over 80% of inherited breast and ovarian cancers. Alternative splicing affects localization and function, and several disease-associated variants have been identified. A related pseudogene is located on chromosome 17.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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