Thermo Fisher Scientific beta Actin Monoclonal Antibody (15G5A11/E2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

View 76 publications 76 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:1,000-1:4,000

View 3 publications 3 publications

Immunoprecipitation (IP)

2 µg

-

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Non-human primate, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

15G5A11/E2

Immunogen

Purified full-length recombinant human beta-actin expressed in E. coli if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta Actin,uniProtId:P60709-1,ncbiNodeId:9606,antigenRange:1-375,antigenLength:375,antigenImageFileName:MA1-140_beta_Actin_P60709-1_House_mouse.svg,antigenImageFileNamePDP:MA1-140_beta_Actin_P60709-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 647
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536844

Product Specific Information

By Western blot, MA1-140 detects a prominent ~42kD protein in human, mouse, non-human primate, and rat whole cell lysates.

For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.