Takara ChIP Elute Kit and DNA SMART ChIP-Seq Kit—Illumina® sequencing library preparation from low-input DNA templates

The ChIP Elute Kit uses a fast and simple method to reverse cross-link, elute, and purify DNA captured by chromatin immunoprecipitation (ChIP). This kit replaces long and tedious protocols for the final stages of ChIP experiments. Single-stranded DNA (ssDNA) can be recovered in approximately one hour (compared to traditional methods that may require overnight incubation) and is directly compatible with the DNA SMART ChIP-Seq Kit.

The ChIP Elute Kit uses a fast and simple method to reverse cross-link, elute, and purify DNA captured by chromatin immunoprecipitation (ChIP). This kit replaces long and tedious protocols for the final stages of ChIP experiments. Single-stranded DNA (ssDNA) can be recovered in approximately one hour (compared to traditional methods that may require overnight incubation) and is directly compatible with the DNA SMART ChIP-Seq Kit.

The DNA SMART ChIP-Seq Kit adds Illumina-specific sequencing adapters to the ends of DNA fragments in a ligation-independent manner. Generating libraries for ChIP sequencing (ChIP-seq) can be difficult due to the small amounts of DNA recovered from ChIP experiments, particularly when using antibodies against low-abundance transcription factors. The DNA SMART ChIP-Seq Kit uses a novel version of SMART template-switching technology—adapted for DNA templates—to generate next-generation sequencing (NGS) libraries for Illumina platforms from 100 pg–10 ng of input DNA. Either ssDNA or double-stranded DNA (dsDNA) from ChIP experiments can be used directly with this kit, making it compatible with DNA recovered with the ChIP Elute Kit or by other methods. This highly sensitive kit generates robust sequencing libraries from low-input DNA samples, with minimal handling, in about four hours.

More Less

Overview

• Fast (one hour) cross-link reversal and elution of ChIP DNA
• Ligation-independent adapter addition for ChIP-seq library preparation
• Flexible DNA inputs: 100 pg–10 ng of either ssDNA or dsDNA
• Generate Illumina-ready sequencing libraries in around 4 hours

There are three versions of this kit available:

• The DNA SMART ChIP-Seq Kit - 12 contains the reagents to generate up to 12 indexed libraries using a single forward indexing primer and 12 reverse indexing primers
• The DNA SMART ChIP-Seq Kit - 48 A can generate up to 48 indexed libraries using 4 forward indexing primers and 12 reverse indexing primers
• The DNA SMART ChIP-Seq Kit - 48 B can generate up to 48 indexed libraries using 4 forward indexing primers and 12 reverse indexing primers

Note: DNA SMART ChIP-Seq Kit - 48 A and DNA SMART ChIP-Seq Kit - 48 B use different sets of forward primers with different index sequences

Interested in more data and FAQs about these products? Visit the NGS Learning Center.

More Information

Applications

ChIP Elute Kit:

• DNA elution and cross-link reversal for qPCR or ChIP-seq applications

DNA SMART ChIP-Seq Kit:

• Sequencing library preparation for ChIP-seq from low-input, fragmented DNA
• Illumina-specific NGS sequencing library production

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.