Takara SMART-Seq v4 Ultra Low RNA Kit for the Fluidigm C1 System—high performance single-cell transcriptome analysis

Overview

• Improved SMART-technology-based chemistry—LNA technology in the SMART-Seq v4 Oligo and improved chemistry lead to better performance (higher sensitivity, greater reproducibility, and more GC-rich genes detected).
• Library construction in 2 days—The cDNA synthesis protocol takes approximately two hours, followed by the eight-hour SMART-Seq v4 script on the C1 instrument. The entire library construction protocol can be completed within two working days.
• New script—The accompanying script is supported for use on both the mRNA Seq IFC and the Open App IFC. The SMART-Seq v4 script is available at the Fluidigm Script Hub. The user manual for this product is available on the Documents tab of this web page.

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More Information

Applications

• cDNA synthesis for mRNA-seq from up to 96 single cells on the Fluidigm C1 System
• cDNA synthesis for qPCR analysis on the Fluidigm Biomark platform prior to Illumina sequencing

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.