Thermo Fisher Scientific MECP2 Polyclonal Antibody
MECP2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ELISA, IP, ChIP, RIP 등 다양한 실험에 사용 가능. 인간 시료에 반응하며, Affinity chromatography로 정제됨. PBS buffer에 보존되어 -20°C에서 보관. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:500–1:1,000 |
| Immunoprecipitation (IP) | 50–100 µg/mL |
| ChIP assay (ChIP) | 5 µg/1×10^6 cells |
| RNA Immunoprecipitation (RIP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Raised against the C-terminal region of human MECP2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide, 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
| RRID | AB_2533879 |
Target Information
MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3, and MBD4) that contain a methyl-CpG binding domain. MECP2 binds specifically to methylated DNA, a key modification in eukaryotic genomes that plays an essential role in mammalian development. It represses transcription from methylated gene promoters and is X-linked, subject to X inactivation. While dispensable in stem cells, MECP2 is critical for embryonic development. Mutations in the MECP2 gene are the primary cause of Rett syndrome, a progressive neurological developmental disorder and a major cause of mental retardation in females.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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