Thermo Fisher Scientific MBD1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

ELISA (ELISA)

1:300-1:1,000

-

ChIP assay (ChIP)

1.5 µg/1x10^6 cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Raised against the human MBD1 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 0.1% BSA

Contains

0.05% sodium azide, 0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

RRID

AB_2533877

Target Information

Methyl-CpG-binding domain protein 1 (MBD1) is a member of a family of mammalian proteins that specifically bind methylated regions of DNA. It is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). MBD1 acts as a transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.