Thermo Fisher Scientific MBD1 Polyclonal Antibody
인간 MBD1 단백질에 특이적인 Rabbit Polyclonal 항체로 Western blot, ELISA, ChIP assay에 적합합니다. 고순도 친화 크로마토그래피로 정제되었으며, PBS/BSA 버퍼에 보존됩니다. 메틸화 DNA 결합 연구 및 전사 억제 기전 분석에 활용 가능합니다.
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Thermo Fisher Scientific MBD1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| ELISA | 1:300–1:1,000 |
| ChIP assay | 1.5 µg / 1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Raised against the human MBD1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 0.1% BSA |
| Contains | 0.05% sodium azide, 0.05% ProClin 300 |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
| RRID | AB_2533877 |
Target Information
Methyl-CpG-binding domain protein 1 (MBD1) is a member of a family of mammalian proteins that specifically bind methylated regions of DNA. It functions as a transcriptional repressor that binds CpG islands in promoters where DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG produced by DNA damage via methylmethanesulfonate (MMS). MBD1 contributes to gene silencing by recruiting AFT7IP, which in turn recruits histone methyltransferase SETDB1. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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