Thermo Fisher Scientific c-Rel Monoclonal Antibody (1RELAH5), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1RELAH5

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• eFluor 660
• PE
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865193

Product Specific Information

Description: The 1RELAH5 monoclonal antibody reacts with mouse c-Rel. c-Rel is a 65 kDa hematopoietic lineage-restricted member of the NF-kB family of transcription factors. In the thymus, c-Rel is upregulated in regulatory T cells (Treg) when compared to other CD4+ thymocytes. c-Rel is believed to play a critical role in thymic Treg (nTreg) development, but is not necessary for TGF beta-mediated T reg (iTreg) differentiation. C-Rel is also involved in proliferation and survival in mature T cells.

Applications Reported: This 1RELAH5 antibody has been reported for use in western blotting.

Applications Tested: This 1RELAH5 antibody has been tested by western blot analysis of mouse splenocytes. This can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA., RELB (604758), NFKB1, and NFKB2. These proteins are related through a highly conserved N-terminal region termed the Rel domain, which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (Belguise and Sonenshein, 2007).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.