Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (eBioP4D1 (P4D1)), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

Assay-Dependent

-

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Species Reactivity

All, Human, Mouse, Rat

Published species

Human, Mouse, Plant, Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

eBioP4D1 (P4D1)

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865191

Product Specific Information

Description: The monoclonal antibody eBioP4D1 (p4D1) recognizes ubiquitin, a small 8Ka protein. Ubiquitin units are linked to proteins via a ubiquitination process, thereby signaling the protein for degradation by the 26S proteosome. The process of ubiquitination plays a role in differentiation, cell cycle progression, apoptosis and disease progression. For example, neuronal inclusions called Lewis bodies from Parkinson patients are rich in ubiquitin.

Applications Reported: This eBioP4D1 (P4D1) antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested: This eBioP4D1 (P4D1) antibody has been tested by immunblotting of treated cell lines. This can be used at less than or equal to 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.