Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (eBioP4D1 (P4D1)), eBioscience
Ubiquitin 단백질을 인식하는 eBioP4D1 단클론 항체로, WB, IHC, IP, ELISA 등 다양한 응용에 적합. 0.5 mg/mL 액상 형태로 제공되며, 높은 순도(>90%)와 낮은 응집도(<10%)를 보유. 세포주 분석 및 단백질 분해 연구용으로 최적화된 항체.
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (eBioP4D1 (P4D1)), eBioscience
Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (eBioP4D1 (P4D1)), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 7 publications |
| Immunohistochemistry (IHC) | Assay-Dependent | - |
| ELISA | - | View 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | All, Human, Mouse, Rat |
| Published Species | Human, Mouse, Plant, Yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioP4D1 (P4D1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865191 |
Additional Formats:
Product Specific Information
The monoclonal antibody eBioP4D1 (P4D1) recognizes ubiquitin, a small 8 kDa protein. Ubiquitin units are attached to proteins via ubiquitination, signaling them for degradation by the 26S proteasome. This process is involved in differentiation, cell cycle progression, apoptosis, and disease development. For instance, Lewy bodies in Parkinson’s disease patients are rich in ubiquitin.
Applications Reported: Suitable for immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.
Applications Tested: Validated in immunoblotting of treated cell lines at ≤2 µg/mL. Antibody titration is recommended for optimal assay performance.
Purity: >90% (SDS-PAGE).
Aggregation: <10% (HPLC).
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ubiquitin is a highly conserved protein that targets cellular proteins for degradation via the 26S proteasome. It is synthesized as a precursor protein forming polyubiquitin chains or as a single ubiquitin fused to another protein.
This gene encodes a fusion protein with ubiquitin at the N-terminus and ribosomal protein S27a at the C-terminus. In yeast, post-translational processing generates free ubiquitin monomer and ribosomal protein S27a, a component of the 40S ribosomal subunit containing C4-type zinc finger domains.
Pseudogenes of this gene exist in the genome. Similarly, ribosomal proteins L40 and S30 are synthesized as fusion proteins with ubiquitin or ubiquitin-like proteins. Multiple alternatively spliced transcript variants encoding the same proteins have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.