Thermo Fisher Scientific Phospho-beta Catenin/gamma Catenin (Tyr142, Tyr133) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:1,000

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho-beta-Catenin (Tyr-142) peptide (without carrier). The antibody detects a 92 kDa protein corresponding to the molecular mass of beta-Catenin on SDS-PAGE immunoblots of Hct116 src-transformed cells treated with pervanadate, but not in control cells. Similar results were observed in pervanadate-treated A431 and human endothelial cells.

Target Information

beta-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of beta-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between alpha- and beta-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of beta-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in beta-Catenin. The Tyr-654 phosphorylation regulates beta-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of beta-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.