Thermo Fisher Scientific Phospho-beta Catenin/gamma Catenin (Tyr489, Tyr480) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-beta-Catenin (Tyr-489) synthetic peptide corresponds to amino acid residues around tyrosine 489 of human beta-Catenin. This peptide sequence is highly conserved in rat and mouse beta-Catenin, and has high homology to the conserved site in gamma-Catenin (Tyr-480).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942894

Product Specific Information

This antibody was cross-adsorbed to phospho-beta-Catenin (Tyr-654) peptide before affinity purification using phospho-beta-Catenin (Tyr-489) peptide (without carrier). The antibody detects 84 and 88kDa* proteins corresponding to the molecular mass of gamma-Catenin and beta-Catenin, respectively, on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but does not detect these proteins in control cells.

Target Information

beta-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of beta-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between alpha- and beta-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of beta-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in beta-Catenin. The Tyr-654 phosphorylation regulates beta-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of beta-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.