Thermo Fisher Scientific Phospho-beta Catenin (Tyr86) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-beta-Catenin (Tyr-86) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 86 of human beta-Catenin. This peptide sequence is highly conserved in rat and mouse beta-Catenin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.125 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942891

Product Specific Information

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho-beta-Catenin (Tyr-86) peptide (without carrier). The antibody detects a 92kDa* protein corresponding to the molecular mass of beta-Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells treated with pervanadate (1 mM for 30 min), but not in control cells.

Target Information

Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.