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카탈로그 번호 | 설명 | 상태 | 단위 | 가격 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
635081 | Takara 635081 iDimerize™ Regulated Transcription System, Each pk | 재고문의 | pk | 2,384,000원 | 2,622,400원 |
다른 상품 둘러보기
iDimerize technology can be used to tightly control transcription activation of target genes using chemically induced dimerization of a DNA binding domain and a transcription activation domain. A key advantage of this iDimerize approach to transcription control is its low background. This is possible because the transcriptional activation domain (AD) is physically separated from the DNA prior to addition of the dimerizer ligand.
iDimerize technology can be used to tightly control transcription activation of target genes using chemically induced dimerization of a DNA binding domain and a transcription activation domain. A key advantage of this iDimerize approach to transcription control is its low background. This is possible because the transcriptional activation domain (AD) is physically separated from the DNA prior to addition of the dimerizer ligand.
Transcription factors are bifunctional proteins that recognize specific DNA sequences near target genes (via the DNA binding domain) and then recruit the transcriptional machinery of the cell to activate transcription (via the transcriptional AD). These two domains can work together to activate transcription even when they are expressed as individual proteins and brought together by the A/C Heterodimerizer ligand.
A/C Heterodimerizer Ligand (AP21967)
The A/C Heterodimerizer is a synthetic, cell-permeable ligand that can be used to induce heterodimerization of two fusion proteins. For example, when used with this kit, the DmrC-tagged transcription AD can be induced to dimerize with the DmrA-tagged DNA binding domain. The A/C Heterodimerizer is identical to AP21967, which was previously supplied by ARIAD Pharmaceuticals Inc.
Regulating transcription
Sequentially transfect your cells of interest with:
- A plasmid which expresses the transcription activation and DNA binding components (pHet-Act1-2)
- Your gene of interest cloned downstream of the PZI-1 inducible promoter (in pZFHD1-2)
The DNA binding component remains bound to the promoter at all times, but it cannot activate transcription until it interacts with the transcription activation component via the DmrA and DmrC domains. When the A/C Heterodimerizer is added, the two components interact, and your gene of interest is transcribed from the PZI-1 promoter.
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