Takara LA Taq DNA polymerase—for long-range PCR

TaKaRa LA Taq DNA Polymerase combines Taq DNA polymerase and a DNA-proofreading polymerase, with 3→5 exonuclease activity, to enable PCR amplification of very long DNA templates (long-range PCR). This mixture of enzymes allows for long and accurate (LA) PCR of targets from a variety of templates, including genomic DNA. LA Taq DNA polymerase is supplied with optimized LA PCR Buffer II (with or without Mg2+) and dNTPs.

TaKaRa LA Taq DNA Polymerase combines Taq DNA polymerase and a DNA proofreading polymerase, with 3’→5’ exonuclease activity, to enable PCR amplification of very long DNA templates (long-range PCR). This mixture of enzymes allows for long and accurate (LA) PCR of targets from a variety of templates, including genomic DNA. LA Taq DNA polymerase is supplied with optimized LA PCR Buffer II (with or without Mg2+) and dNTPs.

The presence of the proofreading polymerase increases fidelity as compared to Taq polymerase alone. Using the LA Taq long-PCR system, routine extensions of 20 kb are possible, and products of up to 48 kb can be obtained for some templates.

Other available versions of LA Taq for long-range PCR include:

• One Shot LA PCR Mix Ver. 2.0—LA Taq enzyme premix with Mg2+ buffer, pre-dispensed in 25 µl-aliquots
• TaKaRa LA Taq DNA Polymerase Hot-Start Version—antibody-meditated hot-start PCR to increase reaction specificity and reduce background
• TaKaRa LA Taq DNA Polymerase with GC Buffer—includes buffers designed to amplify DNA templates with high GC content (GC-rich) or with a significant amount of secondary structure
• LA PCR Kit, Ver. 2.1—includes all reagents plus controls needed for amplification of long DNA templates

Our LA PCR Genome DNA Set includes purified, high-molecular-weight human and E. coli genomic DNA plus appropriate primers for use as PCR controls.

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