Thermo Fisher Scientific Phospho-MYH9 (Ser1943) Polyclonal Antibody
Phospho-MYH9 (Ser1943) 항체로 인간, 생쥐, 랫드 시료에서 NMHC-IIA 인산화를 검출 가능. Western blot 및 ICC/IF에 적합하며, 항원 친화 크로마토그래피로 정제됨. 세포 이동, 접착, 분열 연구에 유용. 연구용 전용 시약.
- 카탈로그번호
- PA5143720
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-NMHC-IIA (Ser-1943) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1943 in human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse NMHC-IIA, but is not found in NMHC-IIB and is not well conserved in NMHC-IIC. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942948 |
Product Specific Information
This antibody was cross-adsorbed to unphosphorylated NMHC-IIA (Ser-1943) peptide before affinity purification using phospho-NMHC-IIA (Ser-1943) peptide (without carrier).
The antibody detects a 200 kDa protein corresponding to NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells.
This reactivity is removed after treatment with lambda phosphatase.
The antibody also detects NMHC-IIA (Ser-1943) phosphorylation in chick fibroblasts.
Target Information
The MYH9 gene, located on chromosome 22q12.3, encodes the heavy chain of non-muscle myosin IIA (NMHC IIA), a critical component of the actin cytoskeleton involved in cell migration, adhesion, division, and maintenance of cell shape.
It spans over 106 kilobases with 41 exons encoding a 1,960 amino acid protein forming a hexameric complex with two heavy chains, two regulatory light chains, and two essential light chains.
Mutations in MYH9 cause autosomal dominant disorders collectively known as MYH9-related diseases (MYH9-RD), including May-Hegglin anomaly, Fechtner syndrome, and Epstein syndrome, often associated with macrothrombocytopenia, hearing loss, renal failure, and cataracts.
MYH9 also plays a key role in hematopoiesis, essential for hematopoietic stem and progenitor cell maintenance. Loss of MYH9 function can lead to severe blood cell deficiencies and bone marrow failure.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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