Thermo Fisher Scientific Phospho-MYH9 (Ser1943) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to unphosphorylated NMHC-IIA (Ser-1943) peptide before affinity purification using phospho-NMHC-IIA (Ser-1943) peptide (without carrier).
The antibody detects a 200 kDa protein corresponding to NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells.
This reactivity is removed after treatment with lambda phosphatase.
The antibody also detects NMHC-IIA (Ser-1943) phosphorylation in chick fibroblasts.

Target Information

The MYH9 gene, located on chromosome 22q12.3, encodes the heavy chain of non-muscle myosin IIA (NMHC IIA), a critical component of the actin cytoskeleton involved in cell migration, adhesion, division, and maintenance of cell shape.
It spans over 106 kilobases with 41 exons encoding a 1,960 amino acid protein forming a hexameric complex with two heavy chains, two regulatory light chains, and two essential light chains.
Mutations in MYH9 cause autosomal dominant disorders collectively known as MYH9-related diseases (MYH9-RD), including May-Hegglin anomaly, Fechtner syndrome, and Epstein syndrome, often associated with macrothrombocytopenia, hearing loss, renal failure, and cataracts.
MYH9 also plays a key role in hematopoiesis, essential for hematopoietic stem and progenitor cell maintenance. Loss of MYH9 function can lead to severe blood cell deficiencies and bone marrow failure.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.