Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Brilliant Violet 480, eBioscience

Applications

• Flow Cytometry (Intracellular staining)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The A1exF5 monoclonal antibody recognizes both human and mouse Arginase 1 (Arg1), a cytosolic enzyme.
Compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis.

Applications Tested:
Tested using intracellular staining of normal human lysed whole blood with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Recommended use ≤ 1.0 µg per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.

Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm and is optimized for cytometers with a 405 nm violet laser.
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) when using multiple polymer dye-conjugated antibodies to reduce non-specific interactions.
BV480 is not compatible with methanol-based fixation.

Excitation: 440 nm
Emission: 479 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, the final step in the urea cycle.
It plays a role in cell proliferation and detoxification, down-regulating nitric oxide production by depriving NO synthase of its substrate.
Expressed in liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells.
In mice, Arginase 1 marks alternatively activated macrophages (M2a).
Associated with hepatocellular carcinoma and argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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