Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Brilliant Violet 480, eBioscience
인간 및 마우스 Arginase 1을 인식하는 A1exF5 클론 단클론 항체로, Brilliant Violet 480 형광체가 결합되어 있습니다. 세포내 염색 및 유세포 분석에 적합하며, 405 nm 레이저 사용 시 최적의 형광 신호를 제공합니다. PBS/BSA 완충액에 보관하며 4°C 암소 저장 권장.
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Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Brilliant Violet 480, eBioscience
Applications
- Flow Cytometry (Intracellular staining)
Tested Dilution: 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Violet™ 480, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A1exF5 |
| Immunogen | E.coli-derived recombinant mouse Arginase 1 |
| Conjugate | Brilliant Violet™ 480 |
| Excitation / Emission Max | 437 / 478 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_3074102 |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The A1exF5 monoclonal antibody recognizes both human and mouse Arginase 1 (Arg1), a cytosolic enzyme.
Compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested using intracellular staining of normal human lysed whole blood with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Recommended use ≤ 1.0 µg per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.
Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm and is optimized for cytometers with a 405 nm violet laser.
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) when using multiple polymer dye-conjugated antibodies to reduce non-specific interactions.
BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm
Emission: 479 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, the final step in the urea cycle.
It plays a role in cell proliferation and detoxification, down-regulating nitric oxide production by depriving NO synthase of its substrate.
Expressed in liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells.
In mice, Arginase 1 marks alternatively activated macrophages (M2a).
Associated with hepatocellular carcinoma and argininemia, an autosomal recessive disorder characterized by hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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