Thermo Fisher Scientific PGP9.5 Polyclonal Antibody
PGP9.5 (UCHL1) 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF, Flow Cytometry에 적합합니다. 인간 및 생쥐 반응성이 있으며, 신경세포 및 신경계 연구에 활용됩니다. 액상 형태로 2 mg/mL 농도, 4°C~−20°C 보관.
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Thermo Fisher Scientific PGP9.5 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 |
| Immunocytochemistry (ICC/IF) | 1:10–1:1,000 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 16–46 amino acids from the N-terminal region of human UCHL1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Size-exclusion - Dialysis, Ammonium sulfate precipitation |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at −20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2210502 |
Product Specific Information
This antibody is predicted to react with bovine and porcine based on sequence homology.
Target Information
PGP9.5 (Protein Gene Product 9.5, UCH-L1, PARK5) is a neuron-specific protein, structurally and immunologically distinct from neuron-specific enolase. It has a molecular weight of 27 kDa and is part of the ubiquitin carboxyl-terminal hydrolase family (peptidase family C12) with a ubiquitin hydrolase domain.
PGP9.5 exhibits both ubiquitin hydrolase and ligase activities, hydrolyzing small C-terminal adducts of ubiquitin to generate monomers. It is expressed in neurons and nerve fibers throughout the central and peripheral nervous systems, neuroendocrine cells, renal tubules, spermatogonia, Leydig cells, ova, and corpus luteum cells.
Overexpression of PGP9.5 is associated with non-small cell lung cancer, while decreased expression is linked to Huntington’s and Alzheimer’s diseases. Due to its presence in neuronal inclusions, PGP9.5 serves as a valuable neuronal marker and is used in studies of neurodegenerative disorders such as Parkinson’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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