Thermo Fisher Scientific Phospho-Connexin 43 (Ser282) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Connexin 43 (GJA1) only when phosphorylated at Ser282.

Target Information

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as hexamers and are transported to the plasma membrane to form hemichannels that connect adjacent cells, creating gap junctions. These structures enable intercellular communication essential for development and regulation of cell growth.

Phosphorylation of Cx43 regulates gap junction assembly and function. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC), reducing cell-to-cell communication. Src kinase can also phosphorylate Cx43, altering junctional communication. Connexin 43 is the predominant gap junction protein in the heart, playing a crucial role in synchronized cardiac contraction and embryonic development. It is regulated by several protein kinases involved in myocardial cell coupling. A pseudogene, GJA1P, is located on chromosome 5. Mutations in related genes can cause disorders such as Charcot-Marie-Tooth disease, oculodentodigital dysplasia, and heart malformations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.