Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (CX5), Functional Grade, eBioscience
마우스 NKG2D 인식용 CX5 클론 단클론 항체로 NK 세포 활성화 및 기능 분석에 적합. Flow cytometry 및 중화·기능성 분석에 사용 가능. 1 mg/mL 액상 형태, 보존제 없음, 4°C 보관. 다양한 형광 접합체 포맷 제공.
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Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (CX5), Functional Grade, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | 26 publications |
| Neutralization (Neu) | Assay-Dependent | 4 publications |
| Functional Assay (Functional) | Assay-Dependent | 2 publications |
| Inhibition Assays (IA) | - | 2 publications |
| Miscellaneous (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CX5 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2866070 |
Available Conjugate Formats
- Alexa Fluor 488
- APC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Custom conjugation available upon request
Product Specific Information
The CX5 monoclonal antibody reacts with mouse NKG2D, a lectin-like molecule expressed on NK cells. Mouse NKG2D binds to RAE-1 (α, β, γ, δ, ε) and H60 molecules, co-stimulating NK activation receptors via DAP12/DAP10 adaptors.
Applications Reported:
- Flow cytometric analysis
- Blocking of NKG2D-ligand binding (RAE-1, H60)
- Inhibition of NK cell-mediated cytotoxicity against ligand-bearing tumors (in vitro, in vivo)
Applications Tested:
- Tested by flow cytometric analysis of mouse splenocytes
- Recommended ≤ 0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test)
- Use Biotin anti-rat secondary antibody (Product #12-5783-81)
- Expression confirmed in C57BL/6 and NWNA mice; not detected in Balb/c or DBA/2
- No cross-reactivity to Ly-49A, C, D, or G2 observed
- 3D10 antibody induces lysis of target cells in culture
Additional Notes:
Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Add 5 µL per sample when staining with NovaFluor conjugates.
For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).
Optical Properties:
- Excitation: 509 nm
- Emission: 614 nm
- Laser: 488 nm (Blue)
NovaFluor conjugates utilize Phiton™ nucleic acid dye technology for engineered fluorescent signatures minimizing spillover and spread.
Target Information
Natural killer (NK) cells mediate lysis of tumor and virus-infected cells without prior activation. They regulate humoral and cell-mediated immunity and express several C-type lectins implicated in NK cell function.
The NKG2 gene family encodes type II transmembrane proteins with C-type lectin domains, located within the NK gene complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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