Thermo Fisher Scientific CD262 (DR5) Monoclonal Antibody (MD5-1), Functional Grade, eBioscience

Thermo Fisher Scientific CD262 (DR5) Monoclonal Antibody (MD5-1), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Biotin
• PE
• Request custom conjugation

Product Specific Information

The MD5-1 monoclonal antibody reacts with mouse DR5 (TRAIL-R2, Apo2, TRICK2, KILLER). DR5 binds to TRAIL, activates NF-kappaB, and induces TRAIL-mediated apoptosis. DR5 mRNA is broadly expressed in normal tissue, and human antigen is also expressed by some tumor cell lines.

Applications Reported:

• Flow cytometric analysis
• Functional assays

Applications Tested:
The MD5-1 antibody has been tested by flow cytometric analysis of mouse DR5-transfected cells. It can be used at ≤0.25 µg per test (100 µL final volume). Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal assay performance.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90%, determined by SDS-PAGE.

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).

Aggregation:
<10%, determined by HPLC.

Target Information

DR5 (Apo2, TRAIL-R2, TRICK2, KILLER) is a death domain-containing receptor for TRAIL, mediating apoptosis. It belongs to the TNF-receptor superfamily and can be activated by TNFSF10/TRAIL/APO-2L. FADD adaptor protein is required for DR5-mediated apoptosis. Two transcript variants and one non-coding transcript exist. Overexpression induces apoptosis and activates NF-kappa-B. DR5 is highly expressed in lymphocytes and spleen. Dysfunction is associated with squamous cell carcinoma and diffuse infiltrative lymphocytosis syndrome.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.