Thermo Fisher Scientific CD273 (B7-DC) Monoclonal Antibody (MIH18), Functional Grade, eBioscience
인간 B7-DC(PD-L2)를 인식하는 MIH18 클론 단일클론 항체로, PD-1 결합 차단 기능을 가짐. Flow cytometry 및 기능적 분석에 적합하며, 고순도(>90%) 및 저엔도톡신 수준(<0.001 ng/µg). 액상 형태로 4°C 보관, 연구용으로 사용.
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Thermo Fisher Scientific CD273 (B7-DC) Monoclonal Antibody (MIH18), Functional Grade, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 8 publications |
| Neutralization (Neu) | - | View 1 publication |
| Functional Assay | Assay-Dependent | View 2 publications |
| T-Cell Activation (TCA) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MIH18 |
| Conjugate | Functional Grade |
| Available Formats | Unconjugated, Alexa Fluor 488, APC, Biotin, PE, PerCP-eFluor 710, Custom conjugation available |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2866079 |
Product Specific Information
Description:
The MIH18 monoclonal antibody reacts with human B7-DC (PD-L2), a member of the B7 family with an approximate molecular weight of 25 kDa. It is primarily expressed by dendritic cells and monocytes/macrophages. Unlike other B7 family members, B7-DC does not bind CD28/CTLA-4 but serves as a ligand for PD-1. MIH18 functions as a blocking antibody and is a valuable tool for studying PD-L2’s role in APC/T cell interactions and expression patterns in humans.
Applications Reported:
- Flow cytometric analysis
- Functional assays
Applications Tested:
Tested on mature dendritic cells and normal human peripheral blood cells. Use ≤0.5 µg per test (100 µL final volume). Cell number per test may vary from 10⁵ to 10⁸ cells and should be optimized empirically.
Storage and Handling:
Use in a sterile environment.
Filtration:
0.2 µm post-manufacturing filtered.
Purity:
90%, determined by SDS-PAGE.
Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).
Aggregation:
<10%, determined by HPLC.
Target Information
Programmed death-ligand 2 (PD-L2/B7-DC):
PD-L2 is a member of the B7 ligand family within the immunoglobulin superfamily and acts as a ligand for PD-1. It is mainly expressed in dendritic cells but can be induced in various immune and non-immune cells depending on cytokine conditions (IL-4, TNF-α, IFN-γ). PD-L2 has 2–6 times higher affinity for PD-1 compared to PD-L1. It plays a dual role in immune regulation—both inhibitory and stimulatory—depending on receptor interactions. PD-L2 also binds to RGMb, influencing respiratory tolerance via BMP/neogenin signaling.
The human PD-L2 monomer consists of a 201-aa extracellular domain, a 21-aa transmembrane domain, and a 32-aa cytoplasmic domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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